Table 2. Changes in brain regions (sampled as Hoechst + cells/section following the procedures outlined underneath the ‘Cell Counting’ subheading in the Materials and methods) sampled across experiments.
All power analyses were performed using G*Power (Peirce, 2008).
| Experiment | Region sampled (Hoechst + cells/Section) |
Significantly different? | Test statistic | P value | Power (1-β error probability) |
|---|---|---|---|---|---|
| Restraint (3–6 dpf) | Forebrain | No | t9 = 1.018 | 0.3351 | 0.104 |
| Pallium | No | t5 = 0.4206 | 0.6915 | 0.054 | |
| Subpallium | No | t5 = 1.685 | 0.1528 | 0.280 | |
| Olfactory Bulb | No | t7 = 0.3330 | 0.7489 | 0.060 | |
| Optic tectum | No | t8 = 1.664 | 0.1347 | 0.300 | |
| Restraint (3–9 dpf | Forebrain | Yes (Control > Restraint) | t11 = 3.890 | 0.0025 | 0.938 |
| Pallium | Yes (Control > Restraint) | t13 = 2.657 | 0.0198 | 0.704 | |
| Subpallium | Yes (Control > Restraint) | t23 = 4.725 | <0.0001 | 0.995 | |
| Exercise (3–6 dpf) | Pallium | No | t8 = 1.260 | 0.2430 | 0.199 |
| Subpallium | No | t7 = 1.435 | 0.1943 | 0.240 | |
| Exercise (3–9 dpf) | Pallium | No | t15 = 1.559 | 0.1397 | 0.310 |
| Subpallium | No | t19 = 0.2109 | 0.8352 | 0.055 | |
| Physical vs. Visual stimulation (3–6 dpf) | Pallium | No | F2,14 = 0.5679 | 0.5792 | 0.122 |
| Subpallium | No | F2,14 = 0.2594 | 0.7751 | 0.082 | |
| AG1478 vs. DMSO (3 dpf) | Pallium | No | t7 = 1.184 | 0.2751 | 0.170 |
| AG1478 vs. DMSO (6 dpf) | Pallium | No | F3,79 = 1.852 | 0.1445 | 0.484 |