Appendix 1—table 4. Effect of sp2 sidechain mutations on phase separation.
Phase separation critical concentration values for the N-terminus (1-236) of human Ddx4 and three mutants, 9FtoA and 14FtoA, as reported in (Nott et al., 2015), and RtoK, where all arginine residues have been mutated to lysine (Brady et al., 2017), as well as for the C-terminus (445-632) of human FMR1 and one mutant with all arginine residues mutated to lysine.
| Sample | Concentration at which phase separation is observed (conditions) |
# F | # R | Total # | Mw (Da) |
|---|---|---|---|---|---|
| Ddx4 1–236 | (24°C, 20 mM Na2PO4 pH 6.5, 100 mM NaCl) | ||||
| WT | ~2 mg/mL | 14 | 24 | 236 | 25430 |
| 9FtoA | ~100 mg/mL | 5 | 24 | 236 | 24745 |
| 14FtoA | ~350 mg/mL | 0 | 24 | 236 | 24364 |
| RtoK | Not observed up to 400 mg/mL | 14 | 0 | 236 | 24758 |
| FMR1 445–632 | (4°C, 20 mM Na2PO4 pH 7.4, 2 mM DTT) | ||||
| WT | ~16 mg/mL | 2 | 28 | 188 | 20573 |
| RtoK | Not observed up to 216 mg/mL | 2 | 0 | 188 | 19789 |