Appendix 1—table 5. Comparison of phase separation prediction and disorder prediction.

Two disorder predictors were tested on matched positive and negative sets to the phase separation predictor, comparing the relative discrimination of known phase-separating and known disordered proteins from the PDB, the human proteome, and the same set of known disordered proteins. AUC values are highlighted in blue for AUC >0.8, and red for AUC <0.7. Error values were obtained by bootstrap analysis.

Positive set	AUC (vs. PDB)	AUC (vs. Human)	AUC (vs. Disprot)
Disopred3 (Disorder Predictor)
Phase Separation Test Set	0.982 ± 0.005	0.72 ± 0.03	0.58 ± 0.02
Disprot Set	0.977 ± 0.007	0.66 ± 0.03	N/A
IUPRED-Long (Disorder Predictor)
Phase Separation Test Set	0.893 ± 0.007	0.70 ± 0.03	0.60 ± 0.02
Disprot Set	0.89 ± 0.01	0.64 ± 0.03	N/A
PScore (Phase Separation Predictor)
Phase Separation Test Set	0.961 ± 0.005	0.88 ± 0.01	0.84 ± 0.01
Disprot Set	0.79 ± 0.02	0.58 ± 0.03	N/A