Fig. 1. Numeric expansion of T cells by AaPC.
(A) Numeric expansion of CD3+ T cells following stimulation with low (10:1) or high (1:2) density of OKT3-loaded K562. Data represented as mean±SD, n=6. (B) Expression of CD4 and CD8 in T cells expanded with AaPC measured by flow cytometry following two stimulation cycles. Data represented as mean±SD, n=6. (C) Numeric expansion of CD4+ and CD8+ T cells following stimulation with AaPC. Data represented as mean±SD, n=6. (D) Ki-67 measured by intracellular flow cytometry 9 days after second stimulation cycle. Data shown as representative histogram and mean±SD, n=3. (D) Viability of cells by flow cytometry for annexin V and PI staining 9 days after second stimulation cycle. Data shown as representative dot plots and mean±SD, n=3* p<0.05, ***p<0.001, ****p<0.0001, two-way ANOVA (Sidak’ss post-test).