Fig. 6. Redirected specificity of T cells for EGFR upon expression of CAR from introduced mRNA.
(A) Cytokine production of CAR+ T cells was measured by intracellular staining and flow cytometry in CD8+ T cells. Data represented as mean±SD, n=3. (B) Specific cytotoxicity of CAR+ T cells was determined by CRA. Data represented as mean±SD, n=3. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, two-way ANOVA (Tukey’s post-test).
