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. Author manuscript; available in PMC: 2018 Mar 14.
Published in final edited form as: Mol Psychiatry. 2017 Sep 12;23(6):1487–1495. doi: 10.1038/mp.2017.179

Figure 4. The human PNKD (L) protein oligomerizes with itself and interacts with the RIMS1 α protein.

Figure 4

A. 239FT cells overexpressing GFP, FLAG-PNKD(L), PNKD(L)-Myc or FLAG-PNKD(L) + PNKD(L)-Myc were lysed for Co-IP. The PNKD(L)-Myc was pulled down by the anti-FLAG magnetic beads only in the presence of the FLAG-PNKD(L).

B. 239FT cells overexpressing GFP, RIMS1α-FLAG, PNKD(L)-Myc or RIMS1α-FLAG+PNKD(L)-Myc were lysed for Co-IP. The PNKD(L)-Myc was eluted by the anti-FLAG magnetic beads only in the presence of the RIMS1α-FLAG.