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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Reprod Domest Anim. 2017 Dec 1;53(2):371–376. doi: 10.1111/rda.13116

Figure 1. Gel Image of Allele Specific Assay Testing for Miniature Schnauzer Mutation c.241C>T; p.R81*.

Figure 1

This gel electrophoresis image demonstrates the allele specific assay results with three dogs of known genotype status. Each dog has two lanes, in which the normal and mutant AMHR2 alleles are separately tested for their presence. The first (left) column for each dog contains primers for the normal allele (indicated with “n”), and the second (right) column for each dog contains primers for the mutant allele (indicated with “a”). From left to right: a carrier dog (presence of both the normal and mutant allele, e.g., heterozygote), a normal/wild-type dog (homozygous for the normal allele), and an affected dog (homozygous for the mutant allele). Where no target allele was present, an additional primer for a 390 base pair product acted as an internal PCR control. The last two columns are template-free water controls for detecting any PCR-mixture contamination.