Table 2.
Primer sequences for AMH and AMHR2 exons
| Gene | Exon | Forward Primer | Reverse Primer | Product Size |
|---|---|---|---|---|
| AMH | ||||
| AMH Ex 1a | CAAGGTCACATCCCAGGAG | CTCCCTTGTCCAGGGTCAC | 320 | |
| AMH Ex 1b | CCCATATAAGCCGGAGCAG | CGAGGAAGGTGTGCTCGTAG | 322 | |
| AMH Ex 1c | CCGGAGGTGAGGTCTCG | CACCTACCTTCCTCCAGGTG | 373 | |
| AMH Ex 1d | CCTGGTGACCCTGGACAA | GCTTAGCACCCCATCTGGA | 334 | |
| AMH Ex 1e | GCTACGAGCACACCTTCCTC | CCCTAGCCAGGAACGTAAGG | 353 | |
| AMH Ex 2 | GTCCCCTAGGCTCCCAGTC | CCTCTCACTTGCACACGTTC | 355 | |
| AMH Ex 3 | CCGGTTGGAATCCTGAAG | GTCATCCGCGTGAAGCAG | 359 | |
| AMH Ex 4 | ACGGTAGGCGCTCAAAGAT | CTACCCCCACCCCCAAC | 289 | |
| AMH Ex 4 b | CCCCGAGCACTAAGCTGTG | GTCTCCAGGAAGGGGTCTG | 330 | |
| AMH Ex 4–5 | TGGACCTCGTGCCCTTC | TTGTTGGCCTGGTAGGTCTC | 758 | |
| AMH Ex 5a | GCTGCTGCTGCTCAAGG | CCTCCGACAGGCTGATGA | 355 | |
| AMH Ex 5b | ACCTACCAGGCCAACAACTG | CTGCTGCTTGCTGCTCTTTA | 308 | |
| AMH Ex 5 d | TGGACCTCGTGCCCTTC | CGACACAGGAGCGCCT | 302 | |
| AMH Ex 5g | TGGACCTCGTGCCCTTC | GGTGGCAGCAGCAGCA | 344 | |
| AMH Ex 5 h | ACCCCGCGACACAGGA | GGCAGAGGGCGAGCAG | 243 | |
| AMH Ex 5 i | CCCTGCTGCTGCTGCT | CAGGTCCACGCTCAGCTC | 392 | |
| AMHR2 | ||||
| AMHR2 Ex 1 F | ACCCACAGTGCCCTATATCTG | ACCCACCCCACTCATTCTTC | 246 | |
| AMHR2 Ex 2 F | CCTGTTTTGTCTCTCCCTTGG | TTTCTCCTCCTCTCCCACTG | 362 | |
| AMHR2 Ex 3 F | GGGGGTTATCCTCTCTGTTCC | TAGGGTGAGAACCAGCCTTG | 356 | |
| AMHR2 Ex 4 F | GTGGGTCCAGAAGCAAGTTC | GTCAGGGGAAGCTGGAGAG | 252 | |
| AMHR2 Ex 5 F | TCTAGAGCCTGAGCCCACTG | ATTACCTGGCGGAAACACAG | 339 | |
| AMHR2 Ex 6a F | AGCTGTGTTTCTCCCAGGTG | TGCAGTTCCAGTACCAGCAG | 349 | |
| AMHR2 Ex 6b F | TGTGTTTCCGCCAGGTAATC | AGCGGATGAATAAGCACGAG | 337 | |
| AMHR2 Ex 6c F | AGCTGTGTTTCTCCCAGGTG | AGCGGATGAATAAGCACGAG | 448 | |
| AMHR2 Ex 7 F | GGGAGATGAGAGCGAGGTC | CCAATTCTTTCCCTTCAAGC | 301 | |
| AMHR2 Ex 8 F | TACGCTGCTGTGTCTGGTTC | TAGGTACTGCCGCACTGTTG | 368 | |
| AMHR2 Ex 9 F | TACGGAGTTTGGGACATTGC | GCAGCAGGAGGTAGGTGGAC | 341 | |
| AMHR2 Ex 10 F | TGTCCACCTACCTCCTGCTG | GGCTGGTCCCAGTAGACAAG | 324 | |
| AMHR2 Ex 11 F | TGTTGAAAACAGGGTAGTGGTG | TAACTGCGCGTTTACAGGTG | 382 | |
| AMHR2 Ex 11b F | AGGACTGTTGGGATGCAGAC | CATCCGACCACCAGATTTATG | 302 | |