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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Gastroenterology. 2017 Dec 9;154(4):1047–1060. doi: 10.1053/j.gastro.2017.11.277

Figure 6. Suppressive activity of TNF-α-producing CD4+CD25+Foxp3+ Treg cells.

Figure 6

(A and B) Expression levels of proteins known to be related with the Treg cell suppression function, including Foxp3 (A) and CD39 (B), were analyzed by flow cytometry. (C) Anti-CD3/CD28-stimulated proliferation of non-Treg CD4+ (left) and CD8+ (right) responder T cells was assessed by measuring the percentage of CFSElo cells in Treg-depleted PBMCs and Treg-reconstituted PBMCs at various ratios. Reconstitution at the original ratio of Treg and non-Treg cells in the peripheral blood is marked as ‘1:1’ and indicated by the arrow. The percentage of suppression was calculated as [1-(% Tresp proliferation in the reconstituted PBMCs/% Tresp proliferation in the Treg-depleted PBMCs)]×100 and was compared between AHA patients (n=11) and healthy controls (n=8). (D) Anti-CD3/CD28-stimulated proliferation of non-Treg CD4+ (left) and CD8+ (right) responder T cells was assessed by measuring the percentage of CFSElo cells in Treg-depleted PBMCs and Treg-reconstituted PBMCs from AHA patients (n=5) and healthy controls (n=5) in the presence or absence of anti-TNF-α blocking antibody. The percentage of suppression was calculated as shown above. Error bars represent standard deviations.