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. Author manuscript; available in PMC: 2019 Feb 3.
Published in final edited form as: Int Arch Allergy Immunol. 2018 Feb 3;175(3):147–159. doi: 10.1159/000484898

Figure 3.

Figure 3

Immunoblotting results illustrated by plots of the serum samples showing the six reactive IgE-binding reactive bands. The reactive bands are represented by arrows and their estimated molecular weight. Panel A illustrates the immunoblot for the negative serum sample. Panel B shows a serum sample reactive to the P130 (gray arrow), P108 (green arrow), P83 (pink arrow), and P56 (purple arrow) IgE-binding protein bands; panel C illustrates a serum sample reactive to the P108 (green arrow), P83 (pink arrow), P56 (purple arrow), and P45 (black arrow); panel D demonstrates a serum sample reactive to the P145 (red arrow), P130 (gray arrow), P108 (green arrow), P83 (pink arrow), and P56 (purple arrow). MW (kDa) = molecular markers; on the left side of the panels we have blotted membranes, and on the right side, we have the blots from Image J Software. The y-axis in the plot represents the intensity of the reactive bands from the WB, illustrated as peaks, while the x-axis represents the distance in the membrane. The peaks in the plot match the bands in the membrane. Proteins (30μg crude MME) were separated in 4 – 15% gradient polyacrylamide gels. Sera dilution 1:400, Conjugate dilution 1:20000.