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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Neurochem Int. 2017 May 18;117:77–81. doi: 10.1016/j.neuint.2017.05.012

Figure 1. p62 KO partially rescues degeneration of Drp1-KO Purkinje neurons.

Figure 1

(A) Control, L7-Drp1-KO, p62-KO and L7-Drp1p62-KO mice were fixed at an age of 3 months. Sagittal sections were cut around the median line of the cerebellum and processed for immunofluorescence microscopy using antibodies against a Purkinje neuron marker, Car8, and p62. The boxed regions are shown enlarged, and the scale bar corresponds to 10 μm. (B) Sagittal sections of the cerebellum from the indicated mice were immunostained using anti-Car8 antibodies. The scale bar corresponds to 1 mm. (C) Quantification of Purkinje neuron density. The number of soma of the Purkinje neurons was measured and normalized relative to the length of the Purkinje cell layer. The values represent the mean ± SEM (n = 6 animals for each genotype). We used the Student's t-test to statistically analyze the difference between the L7-Drp1-KO and L7-Drp1p62-KO mice. *p < 0.05, ***p < 0.001.