Fig. 1. miR-27a inhibits cervical cancer cell proliferation, migration, and invasion and promotes cell apoptosis.

a qRT-PCR for miR-27a mRNA with U6 as a reference. miR-27a is downregulated in cervical cancer cell lines HeLa, SiHa, and C33A compared to normal cervical cells. b Cells were treated with miR-27a agomir. Untreated cells and cells transfected with agomir NC served as controls. Representative images of EdU incorporation assays of HeLa cells. Proliferating nucleus were labeled with EdU (red) while total nuclei were counterstained with Hoechst33342 (blue). c Quantitative analysis of the results of EdU assays of HeLa, SiHa, C33A, and CaSki cells. d Representative images of flow cytometry analysis of CFSE-positive HeLa cells show the cell fractions of sequential generations (depicted by the different colors). e The cell proliferation indexes were calculated according to the CFSE flow cytometry analysis results. The histogram shows the proliferation index in HeLa and SiHa cells transfected with miR-27a agomir versus the control groups. f Representative images of flow cytometry apoptosis assays of HeLa cells. g The histogram shows the quantification of apoptosis assay results in HeLa and SiHa cells. Representative images of invasion and migration assays of HeLa cells. I–J The histograms show the number of invading and migrating cervical cancer cells. ***P < 0.001