Fig. 3. miR-27a functions as a tumor suppressor through repressing TGF-βRI expression and TGF-β signaling.

a–c qRT-PCR analysis of TGF-βRI, TGF-βRII, and SMAD3 expression in cervical cancer cells that were transfected with miR-27a agomir in combination with TGF-βRI expression vector or control vector. d Western blot analysis of TGF-βRI, TGF-βRII, SMAD3, and p-SMAD3 levels in cervical cancer cells that were transfected with miR-27a agomir in combination with TGF-βRI expression vector or control vector. A83-01, a TGF-β signaling inhibitor, serves as positive control. DMSO is solvent control of A83-01 treatment. e–i The co-transfection of miR-27a agomir and TGF-βRI expression vector relieves the tumor inhibition effects mediated by miR-27a agomir. e EdU assays. f CFSE proliferation assays. g Invasion assays. h Migration assays. i Flow cytometry apoptosis assays. ***P < 0.001