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. 2018 Mar 12;8:4318. doi: 10.1038/s41598-018-22438-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reproducability over time of PolyFermS model inoculated with same healthy fecal donor (D3); F3 was operated 15 months after F1. PCoA plots of weighted (A) and unweighted (B) were performed based on the UniFrac distance matrix generated from sequencing V4 region of 16S rRNA genes in samples from donor’s feces and fermentation effluents of F1 (D3), F2 (D4) and F3 (D3). Each circle represents a sample from feces and effluent samples from F1 (D3) (red), F2 (D4) (orange) and F3 (D3) (purple). Bar-plot representation of the mean acetate (blue), propionate (red) and butyrate (green) concentrations (mM) (C) of PolyFermS effluent samples of inoculum reactor (IR) during stable operation phase and connection with experimental reactors (IR_F1: day 1–day 36; IR_F3: day 1–day 23); D3, donor 3; F1, fermentation 1; F2, fermentation 2; D4, donor 4; F3, fermentation 3.