Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 12;8:4374. doi: 10.1038/s41598-018-22307-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Isolation and molecular characterization of KPF1 hmAb. (a) Gating strategy to isolate peripheral blood plasmablasts (CD19 + IgD-CD38 + CD27++) 7 days after immunization. (b) Alignment of KPF1 VH and Vk with presumed germline amino acid sequences. (c) Phylogenic analysis of KPF1 lineage members based on amino acid sequence. Lineage members defined as same heavy chain V and J gene usage, HCDR3 length, and ≥85% HCDR3 similarity. Germline sequence is represented by green diamond, sequences obtained from single-cell plasmablast sequencing are represented by orange squares, the KPF1 mAb sequence is represented by the red square, sequences obtained by MiSeq-based deep sequencing of bulk total B cells are represented by blue squares, inferred intermediate sequences are represented by blue circles. Red line indicates the inferred pathway from germline to KPF1 hmAb. Size of symbols are proportional to the number of identical sequences obtained of an individual lineage member (N = 1–55), with the exception of the germline and KPF1 mAb sequences.