Table 1.
Plasmids and strains used in this study
| Name | Description | References |
|---|---|---|
| Plasmids | ||
| CPOTud | TPI promoter and terminator from S. cerevisiae, POT marker from Schizosaccharomyces pombe (2 μ) | Liu et al. (2012) |
| pAlphaAmyCPOT | α factor leader with α-amylase gene inserted into CPOTud | Liu et al. (2012) |
| pAlphaTrEGCPOT | α factor leader with endoglucanase I gene inserted into CPOTud | Bao et al. (2017) |
| pCP-aGLA | α factor leader with glucan 1,4-α-glucosidase gene inserted into CPOTud | Huang et al. (2015) |
| Strains | ||
| CEN.PK530-6CK | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP PGPD-SEC16 | Bao et al. (2017) |
| YIGS16 | CEN.PK530-6CK with pAlphaAmyCPOT | Bao et al. (2017) |
| YIGCS1 | YIGS16 amdSYM PTEF-GCS1 | This study |
| YIGLO3 | YIGS16 amdSYM PTEF-GLO3 | This study |
| AACK | MATa URA3 HIS3 LEU2 TRP1 SUC2 MAL2-8c tpi1(41-707)::loxP with pAlphaAmyCPOT | Bao et al. (2017) |
| AACK-GCS1 | AACK amdSYM PTEF-GCS1 | This study |
| AACK-GLO3 | AACK amdSYM PTEF-GLO3 | This study |
| YIGLO3GCS1 | YIGS16 PTEF-GLO3 amdSYM PTEF-GCS1 | This study |
| CEN.PK530-6CK + EG | CEN.PK530-6CK with pAlphaTrEGCPOT | This study |
| CEN.PK530-6CK + AGL | CEN.PK530-6CK with pCP-aGLA | This study |
| GLO3 + EG | CEN.PK530-6CK amdSYM PTEF-GLO3 with pAlphaTrEGCPOT | This study |
| GLO3 + AGL | CEN.PK530-6CK amdSYM PTEF-GLO3 with pCP-aGLA | This study |