FIG 2.
5′ and 3′ RACE map of predicted fleQas transcription start site and lengths. Observed 5′ and 3′ ends are shown in relation to expression and AlgU binding at the fleQ locus. Two 5′ ends were observed, one at P. syringae pv. tomato DC3000 genomic coordinate 2140072 and a second that begins at either 2140073 or 2140074 (which cannot be unambiguously determined because of the addition of homopolymeric tails matching the genome sequence). The number of times each 3′ end was observed is indicated in the orange bars, with the depth of shading corresponding to the frequency of observation; the length was calculated based on the most distal 5′ end. The length of the bar is shown to scale, with the other genomic features indicated. The nucleotide coordinates are derived from the P. syringae pv. tomato DC3000 genome sequence (NC_004578.1) (55). RNA- and ChIP-seq data are available in reference 22. The arrow above the fleQ gene indicates the direction of transcription of predicted fleQas molecules.