FIG 1.

c-di-GMP regulates TfoY protein levels. (A) Western blot of PAGE-separated protein isolated from V. cholerae parent strains carrying either the P₁₂₃₄-TL-FULL gfp reporter fusion or the G20U variant. Blots were hybridized with anti-GFP primary antibody and HRP-conjugated secondary antibody and detected using ECL. The blot shown is representative of multiple experiments. (B) Quantification of TfoY-GFP fusion protein present in the lanes depicted in panel A (n = 3 or 4). Relative amounts of protein were quantified for each lane and normalized to the WT Vc2 riboswitch with the P_tac-qrgB* group for each blot (set equal to 1 relative unit of protein). L, low; I, intermediate; H, high. Error bars for all graphs indicate the standard deviation. *, P < 0.05.