FIG 2.

Map of Vc2 riboswitch locus and the relative activity of four tfoY promoters. (A) Map showing the four transcriptional start sites identified for tfoY transcripts and their location on the V. cholerae chromosome. Numbering is relative to the start of the tfoY-coding sequence. Sequences used for transcriptional (TS) reporter fusions described in the text are indicated by bidirectional arrows. (B) Bars depict fluorescence normalized by the optical density at 595 nm for the parent V. cholerae strains carrying the tfoY promoter transcriptional reporters at intermediate c-di-GMP concentrations. The reporter genotype is listed on the x axis and corresponds to the sequence indicated in panel A. “-control” indicates the promoterless gfp vector plasmid. Error bars indicate the standard deviation of the results from three biological replicates.