Figure 6.
Effects of CYP2J2 overexpression on energy expenditure in 16‐month‐old mice. (a) Food intake, (b) VO2, (C) VCO 2, (d) RQ (VCO 2/VO 2), (e) spontaneous activity, and (f) energy expenditure (EE) were measured by Columbus Instruments Comprehensive Lab Animal Monitoring System (CLAMS). (g) Expression of mRNA controlling fatty acid oxidation (FAO) in livers, including PPARα, carnitine palmitoyltransferase‐1α (CPT‐1α), acyl‐coenzyme A oxidase 1 (Acox1), and medium‐chain acyl‐CoA dehydrogenase (MCAD), was measured by real‐time PCR. (h) PPARα, CPT1α, Acox1, and MCAD mRNA levels determined by real‐time PCR from gastrocnemius. Data are shown as means ± SE (n = 8 per genotype). *p < .05, 3 m2J2‐Tr vs. 3 mWT; § p < .05, 16 mWT vs. 3 mWT; §§ p < .01, 16 mWT vs. 3 mWT; # p < .05, 16 m2J2‐Tr vs. 16 mWT; ## p < .01, 16 m2J2‐Tr vs. 16 mWT