Table 1.

Primers used for target and housekeeping genes.

GAPDH	For 5′-CATCAGCAATGCCTCCTGCAC-3′
GAPDH	Rev 5′-GTCATGAGTCCTTCCACGATACCAA-3′
CEA	For 5′-AGGACAGAGCAGACAGCAGAG-3′
CEA	Rev 5′-GGTTCCAGAAGGTTAGAAGTGAGG-3′
CK19	For 5′-CCTGACACCATTCCTCCCTTC-3′
CK19	Rev 5′-CCGACGACTGGCGATAGC-3′
ZEB1	For 5′-GGGAGGAGCAGTGAAAGAGA-3′
ZEB1	Rev 5′-TTTCTTGCCCTTCCTTTCTG-3′
ZEB2	For 5′-AAGCCAGGGACAGATCAGC-3′
ZEB2	Rev 5′-CCACACT CTGTGCATTTGAACT-3′
Snail1	For 5′-GCTGCAGGACTCTAATCCAGA-3′
Snail1	Rev 5′-ATCTCCGGAGGTGGGATG-3′
Snail2	For 5′-TGGTTGCTTCAAGGACACAT-3′
Snail2	Rev 5′-GCAAATGCTCTGTTGCAGTG-3′
Twist1	For 5′-AGCTACGCCTTCTGGTCT-3′
Twist1	Rev 5′-CCTTCTCTGGAAACAATGACATC-3′
Twist2	For 5′-CATGTCCGCCTCCCACTA-3′
Twist2	Rev 5′-GCATCATTCAGAATCTCCTCCT-3′
Vimentin	For 5′-AATCCAAGTTTGCTGACCTCTCTG-3′
Vimentin	Rev 5′-TCATTGGTTCCTTTAAGGGCATCC-3′