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. Author manuscript; available in PMC: 2018 Apr 25.
Published in final edited form as: ACS Nano. 2017 Feb 28;11(4):3456–3462. doi: 10.1021/acsnano.6b07258

Figure 1. Hierarchical organization of engineered proteins and nanoparticles into complex superstructures.

Figure 1

(a) Chemical structure of the arginine functionalized gold nanoparticles ArgNPs, and b, schematic representation of different layers of protein–nanoparticle hierarchical organizations observed in a superstructure. (c) high, and (d) low magnification transmission electron microscopy (TEM) images of the superstructures. Inset in c is TEM image of a single granule in a superstructure, where black and white patches represent ArgNPs and GFP-E10 respectively; red lines represent interparticle center-to-center distance. (e-g) Hierarchical organization of different proteins with ArgNPs: Histone 2A-E10 (e), Cre recombinase E10 (f), and single chain antibody fragment scFv(ErbB2)-E10 (g). h, A tilt-section of a tomogram (see Supplementary movie 1); and i, the 3D visualization of a reconstructed superstructure of GFP-E10:ArgNPs in (h), showing the surface (green) and the inner granules (blue) (also see Supplementary movie 2).