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. 2018 Jan 31;10(2):59. doi: 10.3390/toxins10020059

Figure 4.

Figure 4

Immunoprecipitation (IP) of Stx2 in human serum. Stx2 (5 µg) was added into 100 µL of human serum and incubated for 30 min at 37 °C, and then subjected to IP with an anti-Stx2 antibody. The pure Stx2, HuSAP, and the immune complex eluted from the affinity column were run on the same SDS-PAGE, and immunoblotted with antibodies against Stx2, HuSAP, or a mixture of antibodies against Stx2 and HuSAP, respectively. Lane 1, Protein markers; lane 2, immunocomplex (10 µL); lane 3, pure Stx2 (0.5 µg); lane 4, pure HuSAP (0.1 µg).