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. 2018 Jan 31;10(2):59. doi: 10.3390/toxins10020059

Figure 5.

Figure 5

Immunoprecipitation (IP) of Stx2 in serum from an STEC-infected patient. Serum (100 µL) from an STEC-infected patient was subjected to IP with a monoclonal antibody against the Stx2 B-subunit. The pure Stx2, HuSAP, and the immune complex eluted from the affinity column were run on the SDS-PAGE, and immunoblotted with antibodies against Stx2, HuSAP, or a mixture of antibodies against Stx2 and HuSAP, respectively. Lane 1, immunocomplex (10 µL); lane 2, pure Stx2 (0.5 µg); lane 3, pure HuSAP (0.1 µg). Molecular markers are indicated as kilodalton (kDa) at the left side of the panel.