Skip to main content
. 2018 Feb 7;10(2):72. doi: 10.3390/toxins10020072

Figure 2.

Figure 2

Reversed-phase chromatographic profiles of crab-paralyzing fractions from ion-exchange chromatography. (A,B) Reversed-phase chromatographic profiles of fractions I and II previously separated from cation-exchange chromatography, respectively; (C,D) Reversed-phase chromatographic profiles of fractions III and IV previously separated from anion-exchange chromatography, respectively. Conditions: Hypersil H5 ODS column (4.6 × 250 mm), flow rate 0.8 mL/min, linear gradient from 0 to 80% B in 80 min. Chromatographic fractions showing toxicity to crabs are indicated in the figure (1 to 16); (E,F) Reversed-phase chromatographic purification of fraction number 5. Conditions: Discovery RPC18 HPLC column (4.6 × 250 mm), flow rate of 1 mL/min, gradient from 10 to 20% B in 5 min, followed by 20 to 30% in 50 min. The pure toxin was named PhcrTx2. The dashed line in every chromatographic profile represents the gradient of acetonitrile. The asterisk (*) in (A–F) represent the point where PhcrTx1 elutes in the same chromatographic conditions, according to previous results [6].