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. 2018 Feb 7;10(2):74. doi: 10.3390/toxins10020074

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Activities of the yonT promoter and the sr6 promoter in TY (black), CSEG (grey) and CSE (white) medium over growth. B. subtilis strains generated by integration of the indicated vector fragments into the amyE locus of DB104 were grown in TY, CSEG or CSE medium. In early exponential phase (EP), late exponential phase/transition phase (TP) or stationary phase (SP) samples were withdrawn and used for β-galactosidase measurements. Averages of three independent measurements with standard deviations are shown. Vector pMG16 without a promoter insert yielded in all tested media values between 1 and 3 Miller units.