Table 2.
Plasmids used in this study.
| Plasmid | Description | Reference |
|---|---|---|
| pUCB2 | Shuttle vector of pUC19 and pUB110, NeoR, PhleoR | [38] |
| pDR111 | Vector for IPTG-inducible overexpression and integration into the B. subtilis amyE locus, SpecR | [37] |
| pAPNC213cat | Vector for IPTG-inducible overexpression and integration into the B. subtilis AP locus, CmR | [24] |
| pMG16 | Vector for integration of transcriptional lacZ fusions into B. subtilis amyE locus, SpecR | M. Gimpel, unpublished |
| pGAB1 | Vector for integration of translational lacZ fusions into B. subtilis amyE locus, KanR | S. Brantl, unpublished |
| pMGCR1 | pMG16 with yonT from −500 to +10 * | This study |
| pMGCR6 | pMG16 with yonT from −100 to +10 * | This study |
| pMGCR8 | pMG16 with sr6 from −240 to +50 * | This study |
| pMGCR14 | pMG16 with sr6 from −500 to +50 * | This study |
| pGAY1 | pGAB1 with yonT gene | This study |
| pGAY2 | pGAB1 with yoyJ gene | This study |
| pUCBas | pUCB2 with sr6 gene | This study |
| pUCBYT | pUCB2 with yonT gene | This study |
| pUCBYJ | pUCB2 with yoyJ gene | This study |
| pUCBYU | pUCB2 with yonU gene | This study |
| pAPYT3 | pAPNC213cat with yonT gene (M1Stop) | This study |
| pDRYJ | pDR111 with yoyJ gene | This study |
* with regard to the TSS (transcription start site).