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. 2018 Feb 15;10(2):84. doi: 10.3390/toxins10020084

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antibody potency as assessed by the mouse neutralization assay (MNA). Panel above: relative potency of the BeAb and TeAb compared to CR2, 2G11 and RAZ1 IgG and their combinations. 50 μg of CR2, 2G11 or RAZ1 mAb, 25 μg each of the combination of CR2 + 2G11, 16.6 μg each of the combination of CR2 + 2G11 + RAZ1, 50 μg of BeAb or 50 μg of TeAb were mixed with the indicated amount of BoNT/A1 complex and injected intraperitoneally into cohorts of 10 mice, and the number of mice surviving at 5 days was determined. Panel below: relative potency of the TeAb compared to the combination of 2G11 + CR2 + RAZ1 IgG. 40,000 LD₅₀ of BoNT/A1 was combined with either the TeAb or an equimolar combination of 2G11 + CR2 + RAZ1 IgG at the amount indicated, injected intraperitoneally into cohorts of 10 mice and the number of surviving mice was determined at 5 days.

Antibody potency as assessed by the mouse neutralization assay (MNA). Panel above: relative potency of the BeAb and TeAb compared to CR2, 2G11 and RAZ1 IgG and their combinations. 50 μg of CR2, 2G11 or RAZ1 mAb, 25 μg each of the combination of CR2 + 2G11, 16.6 μg each of the combination of CR2 + 2G11 + RAZ1, 50 μg of BeAb or 50 μg of TeAb were mixed with the indicated amount of BoNT/A1 complex and injected intraperitoneally into cohorts of 10 mice, and the number of mice surviving at 5 days was determined. Panel below: relative potency of the TeAb compared to the combination of 2G11 + CR2 + RAZ1 IgG. 40,000 LD₅₀ of BoNT/A1 was combined with either the TeAb or an equimolar combination of 2G11 + CR2 + RAZ1 IgG at the amount indicated, injected intraperitoneally into cohorts of 10 mice and the number of surviving mice was determined at 5 days.