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. 2018 Feb 15;10(2):84. doi: 10.3390/toxins10020084

Figure 6.

Figure 6

Clearance of ciBoNT/A in mice. Left panel: effect of BeAb or 2G11 + CR2 IgG on ciBoNT/A clearance. 5000 pg of of ciBoNT/A was injected iv into mice, followed 5 min later by iv injection of 10 μg of BeAb or an equimolar combination of mAbs CR2 and 2G11 IgG (5 μg of each). The ciBoNT/A concentration in mouse serum was measured at serial time points beginning 5 min after the BoNT/A injection using meso-scale discovery (MSD) based enzyme-linked immunosorbent assay (ELISA). CiBoNT/A without mAb was used as control. Black square: ciBoNT/A alone; blue triangles: BeAb; green triangles: 2G11 + 2CR2. Right panel: Effect of TeAb or 2G11 + CR2 + RAZ1 IgG on ciBoNT/A clearance. Mice were injected with 5000 pg of ciBoNT/A, an initial blood sample taken at 5 min and then mice injected with 10 μg of TeAb or the mAb combination CR2 + RAZ1 + 2G11, 3.3 μg of each). The BoNT/A concentration in mouse serum was measured at serial time points as described above. ciBoNT/A1 only without mAb was used as control. Square: ciBoNT/A alone; blue circles: TeAb-K; red triangles: 2G11, RAZ1, and CR2 combination. Each data point shown is the average value from three mice per data point.