. 2017 Dec;55(4):519–530. doi: 10.17113/ftb.55.04.17.5138

Table 1. Pancreatic lipase apparent catalytic parameters (v_max, K_m, K_i and K_i´) and IC₅₀ of the hydrolysis of pNPL in the presence of different polyphenolic compounds, capsaicin and orlistat.

Compound	c/µM	v_max/(µM/s)	K_m/µM	K_i/µM	K_i´/µM	IC₅₀/µM
pNPL (control)	11.7-124.2	(0.11±0.03)^a	(1.96±0.20)^ab	n.d.	n.d.	n.d.
Caffeic acid	300.21	(0.10±0.02)^a	(0.30±0.06)^d	(370.2±18.4)^a	(388.2±30.2)^a	(401.5±32.1)^a
p-Coumaric acid	275.00	(0.10±0.01)^a	(0.95±0.10)^c	(205.7±44.1)^b	(290.7±39.7)^b	(170.2±20.6)^b
Quercetin	14.53	(0.10±0.02)^a	(2.15±0.40)^a	(12.0±4.4)^d	(17.9±6.9)^d	(6.1±2.4)^c
Capsaicin	40.47	(0.10±0.02)^a	(1.80±0.19)^b	(66.0±14.3)^c	(50.7±13.0)^c	n.d.
Orlistat	9.68	(0.09±0.03)^a	(0.30±0.05)^d	(16.9±4.8)^d	(19.0±2.8)^d	(4.0±1.0)^c

The data are presented as mean value±standard deviation of triplicate analyses. Different letters in the same row indicate statistically significant values (Fisher’s least significant difference analysis, p≤0.05) with respect to control, or between treatments for dissociation constant of free enzyme and enzyme-substrate complex (K_i and K_i´). K_m=Michaelis-Menten constant, n.d.=not determined

Table 1. Pancreatic lipase apparent catalytic parameters (vmax, Km, Ki and Ki´) and IC50 of the hydrolysis of pNPL in the presence of different polyphenolic compounds, capsaicin and orlistat.

Table 1. Pancreatic lipase apparent catalytic parameters (v_max, K_m, K_i and K_i´) and IC₅₀ of the hydrolysis of pNPL in the presence of different polyphenolic compounds, capsaicin and orlistat.