Table 2. Stern-Volmer and bimolecular quenching constant values of the pancreatic lipase interaction with caffeic acid, p-coumaric acid, quercetin and capsaicin.
Compound | Ksv/(10-4 M -1) | R2 | kq/(10-13 M-1∙s-1) | ∆F/AU |
---|---|---|---|---|
Caffeic acid | (3.7±1.2)ab | 0.9798 | (2.3±0.8)ab | (19.1±1.8)a |
p-Coumaric acid | (2.0±0.6)ab | 0.9089 | (1.3±0.2)ab | (18.7±2.3)a |
Quercetin | (4.5±0.5)a | 0.9669 | (2.8±0.1)a | (18.1±2.0)a |
Capsaicin | (0.40±0.01)c | 0.9885 | (0.25±0.03)c | (6.6±2.2)b |
ΔF corresponds to the difference between registered fluorescence intensity of analyzed compounds (caffeic acid, p-coumaric acid, quercetin and capsaicin) at c=1 and 25 µM. The data are presented as mean value±standard deviation of triplicate analyses. Different letters in the same row indicate statistically significant values (Fisher’s least significant difference analysis, p≤0.05). Ksv=Stern-Volmer quenching constant, kq=bimolecular quenching constant, F=fluorescence intensity