Figure 1.

Timeline of events and taxonomic assignment of reads by the LMAT. A, Timeline of clinical course with relevant laboratory findings listed. Taxonomic classification of reads by LMAT [2] from sonicate fluid with (B) and without (C) microbial DNA enrichment using the MolYsis Basic kit prior to DNA extraction. “Reads too short” indicates reads with <30 k-mers (k = 20). Abbreviations: CRP, C-reactive protein (mg/L); ESR, erythrocyte sedimentation rate (mm/hour); IV, intravenous; LMAT, Livermore Metagenomics Analysis Toolkit; PCR, polymerase chain reaction; PMNs, polymorphonuclear leukocytes; R, right.