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. Author manuscript; available in PMC: 2018 Mar 13.
Published in final edited form as: Immunogenetics. 2008 Aug 6;60(11):655–667. doi: 10.1007/s00251-008-0322-2

Fig. 2.

Fig. 2

Alteration of H47 in KIR2DS5 affects surface expression. A representative flow cytometric analysis of NKL cells transfected with constructs encoding N-terminally HA-tagged, C-terminally V5-tagged KIR. The negative control was a cell transfected with a KIR construct without an HA tag. a In NKL, replacement of H47 in KIR2DS5 (*002) with L47 reduced surface expression while alteration to D47 significantly increased surface expression of KIR2DS5. b The same data shown as a histogram of the HA staining of the transfectants after gating on the V5-positive populations. By Student’s t-test compared to wild type for a representative experiment performed in triplicate: ***p-value<0.001