Figure 9.
Relative expression of TwTPS7v2, TwTPS9v2, TwTPS27v2, and closely related TPSs, as well as triptolide concentration in RNAi suspension cells. (A1) Relative expression of TwTPS7v2, TwTPS9v2, TwTPS27v2, as well as the related TPS-c sub-family members TwCPS2 and TwTPS3v2 in TwTPS7v2&TwTPS9v2-RNAi suspension cells. (A2) Relative expression of TwTPS7v2, TwTPS9v2, and TwTPS27v2, as well as related TPS-b sub-family members TwTPS22v2, TwTPS23v2, TwTPS32 and TwTPS33, in TwTPS27v2-RNAi suspension cells. (B) Presence of recombinant RNAi plasmids, as detected by PCR. (C) Triptolide concentration in RNAi suspension cells. The data represent the averages ± standard deviations of at least four independent lines of suspension cells (* P < 0.05, ** P < 0.01).