Fig 3. Selenium modulates LDL-DHA cytotoxicity.
(A) an overnight incubation of Na2Se (0.05 or 1µM) and (B) 3 days starvation of selenium were applied to H4IIE, PLC/PRF/5 and HepG2 cells, then treated with 40 or 60µM LDL-DHA for 24 hours Cell viability was measured by MTS assay and Lipid peroxidation level was measured by Lipid hydroperoxide assay at 24 hours after LDL-DHA treatment. Results are expressed as mean ± SEM (n=3). *, P <0.05; **, P <0.01; ***, P <0.001 compared with 40µM LDL-DHA only treatment group. #, P <0.05, ##, P <0.01, ###P <0.001 compared with 60µM LDL-DHA only treatment group (one-way ANOVA, Tukey’s test).