Fig. 1.

Impact of the expression of putative P. chrysogenum sugar transporter genes in an l-arabinose metabolizing S. cerevisiae strain in which GAL2 was deleted. Strains were pregrown on liquid SMD and spotted on plates containing 20 g L⁻¹ d-glucose (SMD, left) or l-arabinose (SMA, right) as carbon source. Codes on left-hand side indicate S. cerevisiae strain names and, in brackets, the systematic name of the corresponding over-expressed P. chrysogenum gene. CEN.PK113-7D is a control strain that was not engineered for l-arabinose metabolism. SMD and SMA plates were incubated at 30 °C for 47 and 91 h, respectively. The experiment was performed in duplicate; data shown are from a single representative experiment