Fig. 2.

Growth curves of S. cerevisiae l-arabinose specialist strains, engineered for l-arabinose consumption and disabled for d-glucose consumption by deletion of the hexose kinase genes HXK1, HXK2, GLK1 and GAL1, and expressing either GAL2 (IMX660, filled circles) or the P. chrysogenum transporter PcAraT (IMX869, open circles) as the sole l-arabinose transporter. To assess the ability of Gal2 and PcAraT to support import of l-arabinose by growing cultures in the absence (a) and presence (b) of d-glucose, specific growth rates were estimated from shake flask cultures on synthetic media supplied with 20 g L⁻¹ l-arabinose (a) and on synthetic media supplied with l-arabinose and d-glucose (20 g L⁻¹ each, b)