Table 1.
Saccharomyces cerevisiae strains used in this study
| Strain | Relevant genotype | References |
|---|---|---|
| CEN.PK 113-7D | MATa URA3 HIS3 LEU2 TRP1 MAL2-8c SUC2 | [36] |
| CEN.PK 113-5D | MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2 | [36] |
| CEN.PK102-12A | MATa ura3-52 his3-D1 leu2-3,112 TRP1 MAL2-8c SUC2 | [36] |
| IMX080 | CEN.PK102-12A glk1::SpHis5, hxk1::KlLEU2 | [75] |
| IMX581 | CEN.PK113-5D can1::cas9-natNT2 | [44] |
| IMX486 | IMX080 gal1::cas9-amdSYM | This study |
| IMX604 | IMX486 ura3-52 gre3::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 | This study |
| IMX658 | IMX604 ura3-52 gal80::(pTPI-AraA-tCYC1)*9, pPYK1-AraB-tPGI1, pPGK1-AraD-tTDH3 | This study |
| IMX660 | IMX658 hxk2::KlURA3 | This study |
| IMX728 | IMX658 hxk2::PcaraT | This study |
| IMX844 | IMX660 gal2::KanMX | This study |
| IMX869 | IMX728 gal2::KanMX | This study |
| IMX918 | IMX581 gre3::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 | This study |
| IMX928 | IMX918 gal80::(pTPI-AraA-tCYC1)*9, pPYK-AraB-tPGI1, pPGK-AraD-tTDH3 | This study |
| IMX929 | IMX918 gal80::(pTPI-AraA-tCYC1)*9, pPYK-AraB-tPGI1, pPGK-AraD-tTDH3, pUDE348 | This study |
| IMX1504 | IMX928, gal2Δ, pUDR245 | This study |
| IMX1505 | IMX928 gal2::pADH1-Pc13g04640-tPMA1 (from pPWT111), pUDR245 | This study |
| IMX1506 | IMX928 gal2::pADH1-Pc13g08230-tPMA1 (from pPWT113), pUDR245 | This study |
| IMX1507 | IMX928 gal2::pADH1-Pc16g05670-tPMA1 (from pPWT116), pUDR245 | This study |
| IMX1508 | IMX928 gal2::pADH1-Pc20g01790-tPMA1 (PcaraT) (from pPWT118), pUDR246 | This study |
| IMX1509 | IMX928 gal2::pADH1-Pc22g14520-tPMA1 (from pPWT123), pUDR245 | This study |
| DS68616 | MATa, ura3-52, leu2-112, gre3::loxP, loxP-pTPI-TAL1, loxP-pTPI-RKI1, loxP-pTPI-TKL1, loxP-pTPI-RPE1, leu2::pADH1-XKS1-tCYC1-LEU2, ura3::URA3-pTPI1-XylA-tCYC1 | DSM, The Netherlands |
| DS68625 | DS68616 his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP | [45] |
| DS68625-PcaraT | DS68625, pRS313-PcaraT | This study |
| DS68625-GAL2 | DS68625, pRS313-GAL2 | This study |
| DS68625-mcs | DS68625, pRS313-mcs (empty) | This study |