Table 2.
Plasmids used in this study
| Plasmid | Characteristics | Source |
|---|---|---|
| p414-TEF1p-Cas9-CYC1t | CEN6/ARS4 ampR pTEF1-cas9-tCYC1 | [76] |
| pUG-amdSYM | Template for amdSYM marker | [38] |
| pUG-72 | Template for KlURA3 marker | [77] |
| pUG6 | Template for KanMX marker | [78] |
| pUDE327 | 2 μm, KlURA3, pSNR52-gRNA.HXK2.Y | [79] |
| pUDE335 | 2 μm, KlURA3, pSNR52-gRNA.GRE3.Y | [50] |
| pUDE348 | 2 μm, KlURA3, pSNR52-gRNA.GAL80.Y | This study |
| pUDR246 | 2 μm, KlURA3, pSNR52-gRNA.GAL2.Y pSNR52-gRNA.GAL2.Y | This study |
| pUDR245 | 2 μm, KlURA3, pSNR52-gRNA.GAL2.Y pSNR52-gRNA.GAL2.Y | This study |
| pMEL10 | pSNR52-gRNA.CAN1.Y-tSUP4 | [44] |
| pROS10 | 2 μm, KlURA3, pSNR52-gRNA.CAN1.Y pSNR52-gRNA.ADE2.Y | [44] |
| pUD344 | pJET1.2Blunt TagA-pPGI1-NQM1-TagB | [50] |
| pUD345 | pJET1.2Blunt TagB-pTPI1-RKI1-TagC | [50] |
| pUD346 | pJET1.2Blunt TagC-pPYK1-TKL2-TagF | [50] |
| pUD347 | pJET1.2BluntTagG-pTDH3-RPE1-TagH | [50] |
| pUD348 | pJET1.2Blunt TagH-pPGK1-TKL1-TagI | [50] |
| pUD349 | pJET1.2Blunt TagI-pTEF1-TAL1-TagA | [50] |
| pUD405 | pJET1.2Blunt GAL2 flanked KanMX | This study |
| pPWT111 | ampR KanMX, amdSYM, pADH1-Pc13g04640-tPMA1 | This study |
| pPWT113 | ampR KanMX, amdSYM, pADH1-Pc13g08230-tPMA1 | This study |
| pPWT116 | ampR KanMX, amdSYM, pADH1-Pc16g05670-tPMA1 | This study |
| pPWT118 | ampR KanMX, amdSYM, pADH1-Pc20g01790 (PcaraT)-tPMA1 | This study |
| pPWT123 | ampR KanMX, amdSYM, pADH1-Pc22g14520-tPMA1 | This study |
| pUD354 | pMK-RQ-pTPI1-araA-tADH3 | This study |
| pUD355 | pMK-RQ-pPYK1-araB-tPGI1 | This study |
| pUD356 | pMK-RQ-pPGK1-araD-tTDH3 | This study |
| pRS313-mcs | CEN6, ARSH4, HIS3-pHXT7, tHXT7 | [45] |
| pRS313-PcaraT | CEN6, ARSH4, HIS3, ampR, pHXT7-PcaraT-tHXT7 | This study |
| pRS313-GAL2 | CEN6, ARSH4, HIS3, ampR, pHXT7-GAL2-tHXT7 | This study |