Table 3.
Putative transporter genes that showed higher relative transcript levels in aerobic, l-arabinose-limited chemostat cultures of Penicillium chrysogenum than in corresponding d-glucose- and ethanol-limited cultures
| Gene | Strong similarity to | Relative transcript levels under different nutrient limitations | ||||
|---|---|---|---|---|---|---|
| Glucose | l-Arabinose | Ethanol | Ethanol versus glucose (ratio) | l-Arabinose versus glucose (ratio) | ||
| Pc13g08230 | S. cerevisiae maltose transport protein Mal31 | 13 ± 1 | 664 ± 3 | 17 ± 1 | 1.4 | 53 |
| Pc16g05670 | Neurospora crassa glucose transporter rco-3 | 63 ± 28 | 3176 ± 40 | 69 ± 1 | 1.1 | 51 |
|
Pc20g01790
( PcaraT ) |
Kluyveromyces lactis high-affinity glucose transporter HGT1 | 32 ± 6 | 1415 ± 42 | 46 ± 3 | 1.4 | 44 |
| Pc22g14520 | S. cerevisiae allantoate permease Dal5 | 19 ± 2 | 770 ± 104 | 28 ± 1 | 1.5 | 41 |
| Pc13g04640 | K. lactis high-affinity glucose transporter HGT1 | 29 ± 5 | 971 ± 32 | 53 ± 7 | 1.8 | 34 |
| Pc21g10190 | K. lactis high-affinity glucose transporter HGT1 | 12 ± 1 | 167 ± 26 | 12 ± 1 | 1.0 | 14 |
| Pc12g00190 | Candida albicans ABC transporter CDR4 | 13 ± 2 | 164 ± 24 | 29 ± 2 | 2.2 | 12 |
| Pc14g01680 | Escherichia coli l-fucose permease fucP | 106 ± 14 | 1269 ± 172 | 68 ± 1 | 0.64 | 12.0 |
| Pc21g12210 | Aspergillus nidulans quinate transport protein qutD | 12 ± 0 | 118 ± 1 | 12 ± 1 | 1 | 9.8 |
| Pc06g01480 | S. cerevisiae maltose transport protein Mal31 | 459 ± 85 | 3551 ± 102 | 226 ± 3 | 0.5 | 7.7 |
| Pc13g10030 | S. cerevisiae high-affinity nicotinic acid permease Tna1 | 125 ± 25 | 827 ± 33 | 216 ± 3 | 1.7 | 6.6 |
| Pc21g09830 | K. lactis high-affinity glucose transporter HGT1 | 185 ± 9 | 842 ± 1 | 126 ± 3 | 0.68 | 4.6 |
| Pc16g02680 | S. cerevisiae allantoate permease Dal5 | 80 ± 29 | 360 ± 14 | 113 ± 6 | 1.4 | 4.5 |
| Pc12g05440 | S. cerevisiae maltose transport protein Mal31 | 596 ± 201 | 2633 ± 64 | 104 ± 8 | 0.17 | 4.4 |
| Pc13g15590 | S. cerevisiae glucose permease Rgt2 | 12 ± 1 | 48.0 ± 1.0 | 12 ± 1 | 1 | 4.0 |
| Pc13g06440 | S. cerevisiae high-affinity nicotinic acid permease Tna1 | 66 ± 23 | 225 ± 11 | 48 ± 5 | 0.73 | 3.4 |
P. chrysogenum DS1769 was grown in l-arabinose-, d-glucose-, or ethanol-limited chemostat cultures (dilution rate = 0.03 h−1, pH 6.5, T = 25 °C). Underlined genes were selected for further analysis based on a ≥ 30-fold higher transcript level in l-arabinose-limited cultures than in d-glucose-limited cultures. Data represent average ± mean deviation of globally scaled (target 100) Affymetrix microarrays for independent duplicate chemostat cultures