Table 6.
Comparison of key characteristics of Gal2, PcAraT and heterologous l-arabinose transporters that were previously expressed in S. cerevisiae
| Protein | Origin | Km, ARA [mM] | Vmax, ARA [nmol (g biomass)−1 min−1] | GLC transport | XYL transport | Mechanism | References |
|---|---|---|---|---|---|---|---|
| ScGal2 | S. cerevisiae | 335 ± 21.0 57 ± 11 371 ± 19 |
75 ± 5 2.2 ± 0.3 18 ± 0.8 |
✓ | ✓ | Facilitated diffusion | This study [19] [26] |
| PcAraT | P. chrysogenum | 0.13 ± 0.03 | 5.3 ± 0.2 | ✗ | ✗ | H+ symport | This study |
| SsAraT | Scheffersomyces stipitis | 3.8 ± 1.7 | 0.4 ± 0.1 | ✓ | ✗ | nd | [19] |
| AtStp2 | Arabidopsis thaliana | 4.5 ± 2.2 | 0.6 ± 0.1 | ✗ | ✗ | H+ symport | [19] |
| KmAxt1 | Kluyveromyces marxianus | 263 ± 57 | 57 ± 6 | ✗ | ✓ | Facilitated diffusion | [26] |
| PgAxt1 | Pichia guilliermondii | 0.13 ± 0.04 | 18 ± 0.8 | ✗ | ✓ | H+ symport | [26] |
| AmLat1 | Ambrosiozyma monospora | 0.03* | 0.2 ± 0.0 | ✗ | ✗ | nd | [70, 73] |
| AmLat2 | A. monospora | nd | 4 ± 0 | ✗ | ✗ | nd | [70, 73] |
| NcLat-1 | Neurospora crassa | 58 ± 4 | 1945 ± 50 | ✓ | nd | H+ symport | [30] |
| MtLat-1 | Myceliophthora. thermophila | 29 ± 4 | 172 ± 6 | ✗ | nd | H+ symport | [30] |
nd, not determined; ARA, l-arabinose; GLC, d-glucose; XYL, d-xylose. * Km of AmLat1 was determined as a GFP-fusion protein [73]