Fig. 3.

CD133 expression is associated with cell proliferation in TICs a siRNA mediated gene silencing of CD133 for 72 h in TICs. Results represent mean ±S.E from three independent experiments (n = 3 # p < 0.005). b Effect of CD133 silencing on cell proliferation as determined by BrdU incorporation assay (n = 6 *p < 0.05). c Effect of CD133 silencing on the expression level of cell cycle regulatory genes, CyclinD1 and CyclinA2. Expression level was normalized to scrambled siRNA control (Sc) and represented as relative fold, n = 3 *p < 0.05. d. Immuno-staining for proliferation marker, Ki67 (red) in Scrambled and CD133 silenced TICs. Isotype IgG was used as antibody staining control. Nuclei stained with DAPI (Blue). Scale bar = 25 µm. Images represent four independent experiments