Fig. 1.

Block face photography and digital histology pipeline for MRI-histology co-registration. Brain digitally photographed at various stages of block face sampling including intact brain, coronal slices (a) and extracted regions of interest (b). These images are coupled with images of trimmed surface paraffin embedded blocks (d) and serial digital histology images (e) for mapping (dotted arrow) on MRI modalities (c). Solid arrows represent registrations. Annotations—1: superior frontal gyrus, 2: middle frontal gyrus, 3: medial orbital gyrus, 4: gyrus rectus, 5: olfactory sulcus, white arrow: superior frontal sulcus. Scale bar = 1 cm