Table 1.
Occurrence and distribution of Enterocytozoon bieneusi ITS genotypes and multilocus genotypes (MLGs) in pre-weaned dairy calves on five farms in Shanghai, China
| Farm | Farm ranka | Sampling point | Sample size | No. positive for E. bieneusi (%) | ITS genotype (No.) | MLG (No.) |
|---|---|---|---|---|---|---|
| 1 | D | 1 | 36 | 12 (33.3) | BEB4 (11), Mixed infection (1) | MLG-B1 (2) |
| 2 | 12 | 3 (25.0) | BEB4 (3) | – | ||
| 3 | 46 | 13 (28.3) | BEB4 (13) | MLG-B1 (2), MLG-B2 (1) | ||
| 4 | 25 | 5 (20.0) | BEB4 (5) | MLG-B2 (1) | ||
| Subtotal | 119 | 33 (27.7) | BEB4 (32), Mixed infection (1) | MLG-B1 (4), MLG-B2 (2) | ||
| 2 | A | 1 | 47 | 6 (12.8) | CHN4 (4), Type IV and BEB4 (2) | – |
| 2 | 9 | 3 (33.3) | BEB4 (1), Type IV and BEB4 (2) | MLG-B1 (1) | ||
| Subtotal | 56 | 9 (16.1) | CHN4 (4), BEB4 (1), Type IV and BEB4 (4) | MLG-B1 (1) | ||
| 3 | B | 1 | 112 | 44 (39.3) | J (42), BEB4 (1), CHN15 (1) | MLG-J1 (1), MLG-J2 (1), MLG-J4 (1) |
| 2 | 43 | 17 (39.5) | J (17) | MLG-J3 (1), MLG-J5 (1) | ||
| 3 | 81 | 24 (29.6) | J (24) | MLG-J2 (4) | ||
| 4 | 84 | 19 (22.6) | J (19) | MLG-J2 (1) | ||
| 5 | 69 | 27 (39.1) | J (27) | MLG-J2 (4) | ||
| Subtotal | 389 | 131 (33.7) | J (129), BEB4 (1), CHN15 (1) | MLG-J2 (10), MLG-J1 (1), MLG-J3 (1), MLG-J4 (1), MLG-J5 (1) | ||
| 4 | E | 1 | 29 | 12 (41.4) | J (12) | MLG-J6 (3), MLG-J7 (1), MLG-J8 (1) |
| 2 | 10 | 3 (30.0) | J (3) | MLG-J6 (1) | ||
| Subtotal | 39 | 15 (38.5) | J (15) | MLG-J6 (4), MLG-J7 (1), MLG-J8 (1) | ||
| 5 | C | 1 | 109 | 21 (19.3) | BEB4 (20), J (1) | MLG-B1 (7), MLG-B2 (1) |
| 2 | 97 | 5 (5.2) | BEB4 (5) | – | ||
| Subtotal | 206 | 26 (12.6) | BEB4 (25), J (1) | MLG-B1 (7), MLG-B2 (1) | ||
| Total | 809 | 214 (26.5) | J (145), BEB4 (59), CHN4 (4), Type IV and BEB4 (4), CHN15 (1), Mixed infection (1) | MLG-B1 (12), MLG-J2 (10), MLG-J6 (4), MLG-B2 (3), MLG-J1 (1), MLG-J3 (1), MLG-J4 (1), MLG-J5 (1), MLG-J7 (1), MLG-J8 (1) |
aFarm ranks A-E were ranking scores used to evaluate the hygiene status, animal density and facility condition, with A representing “excellent” and E representing “very poor” (see [35] for details)