Figure 4.

Hsf1 and SAGA precisely colocalize between divergent PICs. (A) Log₂ changes in gene expression in an spt3Δ strain relative to wild type, under acute heat shock conditions (Huisinga and Pugh 2004). Gene classes are separated first by SAGA- vs. TFIID-dominated, then by heat shock-mediated changes in TFIIH occupancy (Induced, Repressed, No change), then by whether Spt3 was induced to bind upon heat shock (≥1.5 fold-change from mock at 3 min of heat shock). (B) Gene averaged plots for Hsf1 (red fill), SAGA (Spt3, blue trace), and TFIIB (Sua7, green filled) with respect to Hsf1 motif midpoint. Data were from 3-min heat shock time point. Genes were not oriented, which results in roughly equivalent levels of TFIIB on either side of Hsf1. (C) Gene-averaged plot of strand-separated ChIP-exo tag 5′ ends for SAGA (Spt3), distributed around −1 and TSS features. The direction of coding transcription is from left to right. Plots of tags mapping to the antisense strand are shown inverted. Data from 3 min of heat shock were used.