Figure 5.

Removing the 5′-triphosphate moiety from gRNA improves CCR5 editing efficiency in primary human CD4⁺ T cells. (A) Indel frequencies at the CCR5 locus were calculated by targeted deep sequencing on day 3 and day 7 post-treatment. (B) Indel frequencies at the CCR5 locus were calculated by targeted deep sequencing on day 25 post-treatment. The percentages of cells positive for surface CD4 (C) and CCR5 protein (D) were determined by flow cytometry. Error bars, SEM; n = 3. Statistical significances were calculated by t-test. (*) P < 0.05, (**) P < 0.01, (n.s.) not significant.