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. 2018 Mar 8;11:1323–1331. doi: 10.2147/OTT.S156582

Figure 2.

Figure 2

CREB inhibits MYCT1 transcription.

Notes: (A) Transcription factor CREB binding sites present in the MYCT1 promoter region. (B) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. (C) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. (D) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. (E) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. (F) Binding of CREB to MYCT1 in vivo. DNA fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. **P<0.01; ***P<0.001.

Abbreviations: IDVs, integrated density values; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.