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. 2018 Feb 28;22(9):2469–2481. doi: 10.1016/j.celrep.2018.02.028

Figure 2.

Figure 2

Phosphoproteomic Analysis of Endometrial Cancer Cell Lines Identifies a Pivotal Role for Akt Signaling in FGFR Inhibitor Resistance

(A) Dendrogram of the hierarchical clustering (Pearson correlation distance metric) of phosphoproteomic signatures obtained through mass spectrometry of MFE-296 cells treated with DMSO, 1 μM PD173074 (PD), or untreated (UT) over 1, 7, and 14 days.

(B) Representation of changes in the phosphoproteome of MFE-296 cells treated with 1 μM PD173074 compared to DMSO controls at 1, 7, and 14 days.

(C) Western blot showing changes in pAkt (Ser473) induced by treatment of MFE-296 cells with 1 μM PD173074 over 14 days. Data are representative of three independent experiments.

(D) Left: H&E staining and Ki67 staining of MFE-296PDR cells (upper) and Ishikawa cells (lower) grown in organotypic cultures for 7 days. Cells were cultured in 1 μM PD173074 with or without 1 μM MK2206. Right: quantitation of cell number and Ki67 positive nuclei.

Data are presented as mean ± SEM. Images are representative of at least three independent experiments. H&E images scale bar, 100 μm; Ki67 images scale bar, 50 μm. ∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01. H&E images are automatically spliced composites.