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. 2018 Jan 5;9(1):4. doi: 10.1038/s41419-017-0076-6

Fig. 5. MiR-494 activates the mTOR pathway and increases resistance to stress conditions.

Fig. 5

a Cell images of miR-494 overexpressing Huh-7 cells or b miR-494 silenced SNU182 cells (10X magnification). c Cell viability assay, caspase-3/7 activity assay and WB analysis in untreated miR-494 overexpressing Huh-7 or d miR-494 silenced SNU182 cells. e FACS Annexin-V plots of miR-494 overexpressing and control untreated Huh-7 (following 48 h of transfection). f Cell viability assay, caspase-3/7 activity assay, and WB analysis in miR-494 overexpressing Huh-7 or g miR-494 silenced SNU182 cells in starvation condition. h FACS Annexin-V plots of miR-494 overexpressing and control Huh-7 following 24 h of starvation. NC: pre-miR negative control. NCi: anti-miR negative control, AM-494: anti-miR-494. β-actin was used to normalize WB data