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. Author manuscript; available in PMC: 2018 Mar 13.
Published in final edited form as: Cell Rep. 2018 Feb 20;22(8):2118–2132. doi: 10.1016/j.celrep.2018.01.077

Figure 2. Differentiation of Cry1−/− and Cry2−/− Myoblasts.

Figure 2

(A) Immunostaining of primary myoblasts prepared from WT, Cry1−/−, and Cry2−/− mice with antibodies against MHC and MyoD. Cells were induced to differentiate with 5% HS for 1 and 3 days. Scale bar, 50 μm.

(B–D) Frequency of nuclei within MyoD(+) or MHC(+) cells among 500 total nuclei on day 0 (B), day 1 (C), and day 2 (D).

(E and F) Fusion index (E) and < 3 nuclei index (F) on day 2, based on observation of 500 nuclei.

Data are presented as mean + SD.